Transformation of enterprise: chongqing three hang seng engineering co., LTD
Project leader: jin-hua wang transformation amount: 6 million
Content abstract: lactic acid is widely regarded as one of the three organic acids in the world, by its polymerization of poly lactic acid (poly real lactic acid, PLA) manufactured products have good biocompatibility, and have certain bacteria resistance, flame retardant and ultraviolet resistance, so the lactic acid and its derivatives in brewing, food, agriculture, medicine, chemical industry, leather, tobacco, printing and dyeing, textile and other industries widely used.The research team used Red homologous recombination technology, non-trace knock out wild type escherichia coli other competitive metabolites (ethanol, methanol, succinic acid, acetic acid) of key genes, built to produce high purity D - lactic acid bacteria.After the domestication of the bacteria for metabolism, when using sucrose as a carbon source for fermentation, lactic acid yield is up to 88.15 g/L, D - lactic acid the optical purity of 99%.Basic no miscellaneous acid in the fermented liquid by-products generated, such as the separation of lactic acid.The fungus for the first available sucrose D lactic acid fermentation engineering bacterium e. coli.Constructed strain of the optical purity of the product production reached the international advanced level.Build strains by the scientific research achievements, can use cheap inorganic salt medium, use sucrose as carbon source, greatly reducing the costs of industrialized production.The lactic acid optical purity can reach 99.5%, reached the international advanced level.
The results by the department of hubei province in 2012 identified as the international advanced level.