Transformation of enterprises: the angel yeast co., LTD
Project leader: Cai Jun
Content abstract: glutathione in medicine, food and other fields have a wide range of use and important function.Glutathione on clinical diagnosis and medicine can be used as free radical scavenger, protect cells, remove some toxicity of exogenous toxic substances to the body;Promote cell protein synthesis;Participate in c amino methyl, turns, maintain the normal cell functions;Participate in bilirubin metabolism;Promote the metabolism of bile acid, reduce bleeding tendency;Inhibit the development of cataract and corneal and diseases of the retina;Prevent the skin pigmentation in the melanin formation and reduce its oxidation;Relieve symptoms after radiation and so on.Glutathione reduction function can a add flour products, effectively prevent the barley powder, flour, dairy products that are rich in protein and enzymatic of fruits, vegetables, food or non enzymatic Browning, and also can strengthen the amino acids, enhance the effect of food flavor and nutrition, improve the quality of the dough.
Project with high yield and glutathione saccharomyces cerevisiae Y - 14 strains, using shake flask fermentation process was optimized and the composition of culture medium, studies the technologies of fermentation metabolic regulation, the high yield and glutathione fermentation conditions were determined and metabolic regulation method;On this basis, in the 30 L automatic tank fermentation experiments, 2 m after pilot pot fermentation experiment, 60 m after the big test, the system optimizes the saccharomyces cerevisiae Y - 14 in molasses and glucose for composite carbon source, the flow and fermentation process in the production of glutathione fermentation technology conditions, medium composition, flow and strategy, the fermentation process is studied in dissolved oxygen concentration, concentration of reducing sugar and ethanol content of fermentation in the production of glutathione, determined the best control point, implement the efficient synthesis of glutathione fermentation technology, is fermented liquid per liter of yeast cell dry weight reached more than 60 grams, intracellular GSH content reached more than 8 g / 100 g, GSH content in the fermented liquid above 4800 mg/L.System studied the separation and purification of GSH method and technological conditions, the results show that the separation and purification of GSH yield was 76.46%, the purity of product was 92.40%.Rich in glutathione yeast products indicators reached the enterprise standard Q/YB2227S - 2013.The product is used for improved this dough with DSM (DSM) products.